morphine-induced analgesic tolerance is associated with alteration of protein kinase cγ and transient receptor potential vanilloid type 1 genes expression in rat lumbosacral cord and midbrain

نویسندگان

shamseddin ahmadi department of biological science and biotechnology, faculty of science, university of kurdistan, sanandaj, iran

neda parvini department of biological science and biotechnology, faculty of science, university of kurdistan, sanandaj, iran

چکیده

introduction: transient receptor potential vanilloid type 1 (trpv1) and protein kinase cγ (pkcγ) are involved in sensitization/desensitization to noxious stimuli. we aimed to examine the gene expression levels of trpv1 and pkcγ in rat lumbosacral cord and midbrain on days 1, 4 and 8 of induction of morphine analgesic tolerance. methods: two groups of male wistar rats received twice daily saline (1 ml/kg) or morphine (10 mg/kg) for eight days and were monitored for analgesic tolerance with a hotplate test on days 1, 4 and 8 of the injections. six independent groups in three sets were also treated with saline or morphine, decapitated on days 1, 4 or 8 of the schedule, respectively and their lumbosacral cord and midbrain were dissected. results: the result of the hotplate test revealed induction of analgesic tolerance on days 4 and 8 of morphine injections. the trpv1 gene expression in the lumbosacral cord was significantly increased only on day 4 of morphine injections, but the pkcγ gene expression remained with no significant changes on days 1, 4 and 8. in the midbrain, the trpv1 gene expression was significantly increased only on day 1; however, the pkcγ gene expression was significantly increased on days 4 and 8 of morphine injections. conclusion: it can be concluded that the trpv1 gene expression changes in the lumbosacral cord and midbrain is associated with early phase of morphine-induced analgesic tolerance but the pkcγ gene expression is altered only in midbrain at the later phase of process.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Morphine-induced analgesic tolerance is associated with alteration of protein kinase Cγ and transient receptor potential vanilloid type 1 genes expression in rat lumbosacral cord and midbrain

Introduction: Transient receptor potential vanilloid type 1 (TRPV1) and protein kinase Cγ (PKCγ) are involved in sensitization/desensitization to noxious stimuli. We aimed to examine the gene expression levels of TRPV1 and PKCγ in rat lumbosacral cord and midbrain on days 1, 4 and 8 of induction of morphine analgesic tolerance. Methods: Two groups of male Wistar rats received ...

متن کامل

Association of morphine-induced analgesic tolerance with changes in gene expression of GluN1 and MOR1 in rat spinal cord and midbrain

Objective(s): We aimed to examine association of gene expression of MOR1 and GluN1 at mRNA level in the lumbosacral cord and midbrain with morphine tolerance in male Wistar rats. Materials and Methods: Analgesic effects of morphine administrated intraperitoneally at doses of 0.1, 1, 5 and 10 mg/kg were examined using a hot plate test in rats with and without a history of 15 days morphine (10 mg...

متن کامل

Morphine-Induced Analgesic Tolerance Effect on Gene Expression of the NMDA Receptor Subunit 1 in Rat Striatum and Prefrontal Cortex

Introduction: Morphine is a potent analgesic but its continual use results in analgesic tolerance. Mechanisms of this tolerance remain to be clarified. However, changes in the functions of μ-opioid and N-Methyl-D-aspartate (NMDA) receptors have been proposed in morphine tolerance. We examined changes in gene expression of the NMDA receptor subunit 1 (NR1) at mRNA levels i...

متن کامل

association of morphine-induced analgesic tolerance with changes in gene expression of glun1 and mor1 in rat spinal cord and midbrain

objective(s): we aimed to examine association of gene expression of mor1 and glun1 at mrna level in the lumbosacral cord and midbrain with morphine tolerance in male wistar rats. materials and methods: analgesic effects of morphine administrated intraperitoneally at doses of 0.1, 1, 5 and 10 mg/kg were examined using a hot plate test in rats with and without a history of 15 days morphine (10 mg...

متن کامل

gene expression profile of calcium/calmodulin-dependent protein kinase iiα in rat spinal cord and midbrain during induction of morphine analgesic tolerance

background calcium/calmodulin-dependent protein kinase iiα (camkiiα) may modulate the function of mu-opioid receptors by phosphorylation and therefore, be involved in development of morphine-induced analgesic tolerance. objectives the current study aimed to examine changes in gene expression of camkiiα in the lumbosacral cord and midbrain during induction of morphine analgesic tolerance. materi...

متن کامل

Effects of capsazepine, a transient receptor potential vanilloid type 1 antagonist, on morphine-induced antinociception, tolerance, and dependence in mice.

BACKGROUND Repeated morphine treatment has been shown to induce transient receptor potential vanilloid type 1 (TRPV1) expression in the spinal cord, dorsal root ganglion (DRG), and sciatic nerve of a rat model. Increased TRPV1 expression may therefore play a role in morphine tolerance. In this study, we evaluated the hypothesis that blockage of TRPV1 may be useful as an adjunctive pain manageme...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
physiology and pharmacology

جلد ۲۰، شماره ۳، صفحات ۱۴۷-۱۵۶

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023